LOCUS       sequence-165765-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human TP53 around codon F109.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, 
            Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, 
            Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, 
            Grumolato L
  TITLE     CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and 
            Functional Analysis of Oncogenic Driver Mutations.
  JOURNAL   Mol Cell. 2016 Aug 4;63(3):526-38. doi: 
            10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21.
  PUBMED    27453044
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.06.017"; journalName: "Mol Cell"; date: 
            "2016-08-4- 4"; volume: "63"; issue: "3"; pages: "526-38"
COMMENT     Sequence Label: sgTP53(A)
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     137..156
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        235..310
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgcatcgata catgctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
       61 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
      121 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      181 ggctttatat atcttgtgga aaggacgaaa caccgggcag ctacggtttc cgtcgtttta
      241 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      301 gagtcggtgc ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 tttagcgcgt gcgccaattc tgcagacaaa tggctctaga ggtacccgtt acataactta
      421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa
      481 cgccaatagg gactttccat
//
