LOCUS       sequence-165776-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, 
            Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, 
            Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, 
            Grumolato L
  TITLE     CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and 
            Functional Analysis of Oncogenic Driver Mutations.
  JOURNAL   Mol Cell. 2016 Aug 4;63(3):526-38. doi: 
            10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21.
  PUBMED    27453044
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.06.017"; journalName: "Mol Cell"; date: 
            "2016-08-4- 4"; volume: "63"; issue: "3"; pages: "526-38"
COMMENT     Sequence Label: sgAAVS1(A)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        226..301
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        398..683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 caccgctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccgtccc ctccacccca cagtggtttt agagctagaa
      241 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      301 cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg
      361 tgcgccaatt ctgcagacaa atggctctag aggtacccgt tacataactt acggtaaatg
      421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
      481 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      541 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      601 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      661 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      721 tctccccccc ctccccaccc ccaattttgt at
//
