LOCUS       sequence-165780-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            mRFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB555
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..162
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    231..355
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(408..413,414..431,432..438)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(558..577)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc
       61 actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga gcgcgccgag ggccgccact ccaccggcgc cgctgttatc acaagtttgt
      241 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      301 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
      361 ccctcgaggc ctcgaggcct cgaggggccg cattaggcac cccaggcttt acactttatg
      421 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
      481 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      541 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      601 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      661 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
      721 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      781 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      841 tacacatata ttcgcaagat gtggcgtgtt acggtga
//
