LOCUS       sequence-165783-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            G3GFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB452
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     555..583
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(636..682)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(671..691)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat
       61 tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga
      121 agttaacttt gattccattc ttttgtttgt ctgccatgat gtacacgttg tgggagttga
      181 agttgtattc caacttgtgg ccgaggatgt ttccgtcctc cttgaaatcg attcccttaa
      241 gctcgatcct gttgacgagg gtgtctccct caaacttgac ttcagcacgt gtcttgtagt
      301 tcccgtcgtc cttgaagaag atggtcctct cctgcacgta tccctcaggc atggcgctct
      361 tgaagaagtc gtgccgcttc atatgatctg ggtatcttga gaagcactgc acaccgtaag
      421 cgaaagtagt aacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt
      481 taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt
      541 gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt
      601 ctcctttact catgctcatt ctagagtccc ccgtgttctc tccaaatgaa atgaacttcc
      661 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
      721 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
      781 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tc
//
