LOCUS       sequence-16579-e        1368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD21_28
FEATURES             Location/Qualifiers
     source          1..1368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(247..269)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngnntgcgg tcgactctag aggatccccg ggattggcca aaggacccaa aggtatgttt
       61 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggaggg taccgagctc
      121 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtgggtgaag accagaaaca
      181 gcacctcgaa ctgagccgcg atattgccca gcgtttcaac gcgctgtatg gcgagatcga
      241 tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      301 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      361 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
      421 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
      481 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
      541 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
      601 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
      661 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
      721 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
      781 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
      841 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
      901 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
      961 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
     1021 cgccagcggc accgcgcctt tcggggggaa attatcgatg ancgtggngg ttatgccgat
     1081 cgcgtccact acgtctgaan gtcaaaancc naantgggga gnncnaaatc cnaatnttnt
     1141 cngggggggt nnantgcnnc ccccaangnc cctnatnaaa naaanctgca tttcgtttcc
     1201 ngagggngaa tnaaangntt gnnntgtnaa ggnaaccttt ntatnnaggn taacnnccaa
     1261 aatttcttng ttggtcgnnt ggnaaanaaa agggggaatc tgtnaaaaaa aaanttancn
     1321 ggggttntat ncnancccng gnaactggga nccccccgtg gggnaana
//
