LOCUS       sequence-165794-        4247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4247)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..4247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2772..2781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2778..4208
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagctca agcttcgcac catcatggac cattatgatt
       61 ctcagcaaac caacgattac atgcagccag aagaggactg ggaccgggac ctgctcctgg
      121 acccggcctg ggagaagcag cagagaaaga cattcacggc atggtgtaac tcccacctcc
      181 ggaaggcggg gacacagatc gagaacatcg aagaggactt ccgggatggc ctgaagctca
      241 tgctgctgct ggaggtcatc tcaggtgaac gcttggccaa gccagagcga ggcaagatga
      301 gagtgcacaa gatctccaac gtcaacaagg ccctggattt catagccagc aaaggcgtca
      361 aactggtgtc catcggagcc gaagaaatcg tggatgggaa tgtgaagatg accctgggca
      421 tgatctggac catcatcctg cgctttgcca tccaggacat ctccgtggaa gagacttcag
      481 ccaaggaagg gctgctcctg tggtgtcaga gaaagacagc cccttacaaa aatgtcaaca
      541 tccagaactt ccacataagc tggaaggatg gcctcggctt ctgtgctttg atccaccgac
      601 accggcccga gctgattgac tacgggaagc tgcggaagga tgatccactc acaaatctga
      661 atacggcttt tgacgtggca gagaagtacc tggacatccc caagatgctg gatgccgaag
      721 acatcgttgg aactgcccga ccggatgaga aagccatcat gacttacgtg tctagcttct
      781 accacgcctt ctctggagcc cagaaggcgg agacagcagc caatcgcatc tgcaaggtgt
      841 tggccgtcaa ccaggagaac gagcagctta tggaagacta cgagaagctg gccagtgatc
      901 tgttggagtg gatccgccgc acaatcccgt ggctggagaa ccgggtgccc gagaacacca
      961 tgcatgccat gcaacagaag ctggaggact tccgggacta ccggcgcctg cacaagccgc
     1021 ccaaggtgca ggagaagtgc cagctggaga tcaacttcaa cacgctgcag accaagctgc
     1081 ggctcagcaa ccggcctgcc ttcatgccct ctgagggcag gatggtctcg gacatcaaca
     1141 atgcctgggg ctgcctggag caggtggaga agggctatga ggagtggttg ctgaatgaga
     1201 tccggaggct ggagcgactg gaccacctgg cagagaagtt ccggcagaag gcctccatcc
     1261 acgaggcctg gactgacggc aaagaggcca tgctgcgaca gaaggactat gagaccgcca
     1321 ccctctcgga gatcaaggcc ctgctcaaga agcatgaggc cttcgagagt gacctggctg
     1381 cccaccagga ccgtgtggag cagattgccg ccatcgcaca ggagctcaat gagctggact
     1441 attatgactc acccagtgtc aacgcccgtt gccaaaagat ctgtgaccag tgggacaatc
     1501 tgggggccct aactcagaag cgaagggaag ctctggagcg gaccgagaaa ctgctggaga
     1561 ccattgacca gctgtacttg gagtatgcca agcgggctgc acccttcaac aactggatgg
     1621 agggggccat ggaggacctg caggacacct tcattgtgca caccattgag gagatccagg
     1681 gactgaccac agcccatgag cagttcaagg ccaccctccc tgatgccgac aaggagcgcc
     1741 tggccatcct gggcatccac aatgaggtgt ccaagattgt ccagacctac cacgtcaata
     1801 tggcgggcac caacccctac acaaccatca cgcctcagga gatcaatggc aaatgggacc
     1861 acgtgcggca gctggtgcct cggagggacc aagctctgac ggaggagcat gcccgacagc
     1921 agcacaatga gaggctacgc aagcagtttg gagcccaggc caatgtcatc gggccctgga
     1981 tccagaccaa gatggaggag atcgggagga tctccattga gatgcatggg accctggagg
     2041 accagctcag ccacctgcgg cagtatgaga agagcatcgt caactacaag ccaaagattg
     2101 atcagctgga gggcgaccac cagctcatcc aggaggcgct catcttcgac aacaagcaca
     2161 ccaactacac catggagcac atccgtgtgg gctgggagca gctgctcacc accatcgcca
     2221 ggaccatcaa tgaggtagag aaccagatcc tgacccggga tgccaagggc atcagccagg
     2281 agcagatgaa tgagttccgg gcctccttca accactttga ccgggatcac tccggcacac
     2341 tgggtcccga ggagttcaaa gcctgcctca tcagcttggg ttatgatatt ggcaacgacc
     2401 cccagggaga agcagaattt gcccgcatca tgagcattgt ggaccccaac cgcctggggg
     2461 tagtgacatt ccaggccttc attgacttca tgtcccgcga gacagccgac acagatacag
     2521 cagaccaagt catggcttcc ttcaagatcc tggctgggga caagaactac attaccatgg
     2581 acgagctgcg ccgcgagctg ccacccgacc aggctgagta ctgcatcgcg cggatggccc
     2641 cctacaccgg ccccgactcc gtgccaggtg ctctggacta catgtccttc tccacggcgc
     2701 tgtacggcga gagtgacctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     2761 atccaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
     2821 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     2881 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     2941 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
     3001 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
     3061 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
     3121 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
     3181 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     3241 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
     3301 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
     3361 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     3421 acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca
     3481 gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc aaagagttca
     3541 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
     3601 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     3661 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
     3721 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
     3781 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
     3841 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
     3901 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     3961 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     4021 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     4081 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     4141 aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt
     4201 acaagtaagc ggccgcgact ctagatcata atcagccata ccacatt
//
