LOCUS       sequence-165795-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOT induced by acetoacetate to express GFP. Contains the native 
            atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP
            gene. LOG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoK-LOG241
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctctgtcg accaactagt gccgtgcatt gatgtataaa ctccaggaat acggtatcga
       61 tccggcggat gtataacacc aaaacttgct atgcagaaat ttgcacagtg cgcaattttc
      121 tgcatagccg ctcattctcc ttataaatcc ccatccaatt tatcccttca tattcaatta
      181 gttaaataac taaatccaat aatctcattc tggcactccc cttgctattg cctgactgta
      241 cccacaacgg tgtatgcaag agggataaaa aatgaaaaca aaattgatga cattacaaga
      301 cgccaggtac ccaccatgac agctctgaca gagggagcga aactgttcga gaaggagatc
      361 ccgtacatca cagagttgga aggagatgtc gaagggatga aattcatcat caagggagaa
      421 ggaacgggtg acgcaacgac cggaacgatc aaggccaaat acatttgtac cacgggcgat
      481 ttgcctgtcc cctgggcgac gcttgtaagc acgctctcgt atggtgtcca gtgcttcgcg
      541 aaatatccat cgcacattaa ggactttttc aagtcggcca tgccagaagg ttacacacaa
      601 gaacgaacca tctcctttga gggggacgga gtgtataaga cacgcgcgat ggtaacgtac
      661 gagcgcgggt ccatctacaa cagggtaact cttactgggg agaactttaa gaaggacggg
      721 catatcttgc ggaaaaacgt ggcatttcaa tgtccgccct cgattctgta tattctcccg
      781 gacacggtga ataatgg
//
