LOCUS       sequence-16580-e        1376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD22_04
FEATURES             Location/Qualifiers
     source          1..1376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             136..156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(246..268)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngnctgcng tcgactctag aggatccccg ggattggcca aaggacccaa aggtatgttt
       61 cgaatgatac taacataaca tagaacattt tcaggaggac ccttgagggt accgagctca
      121 gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tgggtgaaga ccagaaacag
      181 cacctcgaac tgagccgcga tattgcccag cgtttcaacg cgctgtatgg cgagatcgat
      241 cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      301 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      361 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
      421 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
      481 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
      541 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
      601 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
      661 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
      721 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
      781 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
      841 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
      901 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcanatgtgc
      961 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga ancgcaggtc
     1021 gccagcggca ccgcgccntt tcggcggtga aattatcgat gancgnggng gttatgccga
     1081 tcgcgtccna ttacgtctga acgtcnaaaa cccgaaactg ngganggncn aaatcccgaa
     1141 tctttntcgn gggggggtna antgccnccc ccccaangcn ncctnattna aaaaaancct
     1201 gnnatnttcg tttnccnnag gggggaatnn aaangnnttt ttnttttnaa aaaaaaccnt
     1261 tttttntnag ggntaacccc ccnaaaattt cttntggngn gggntgggga aaaaaaaagn
     1321 ggggaatttt ttganaaaaa aaantnngng gggnttttnt naaaancccc ggggga
//
