LOCUS       sequence-165805-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged OSBP PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Levine TP, Munro S
  TITLE     The pleckstrin homology domain of oxysterol-binding protein 
            recognises a determinant specific to Golgi membranes.
  JOURNAL   Curr Biol. 1998 Jun 18;8(13):729-39.
  PUBMED    9651677
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "1998-06-18"; volume: "8(13)"; pages: "729-39"
COMMENT     Sequence Label: pRS406-PHO5-GFP-OSBP PH domain
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(245..264)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(492..511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(493..511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(521..538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(521..537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgcaggaatt ctcgaatttg cttgctctat ttgttgttgt tcttattctc atgagagatg
       61 aagccatact aacctcgact tagcaaaaca tcagcgctta tatacgtttc atttcgacaa
      121 ttcaaagatg tttaccctta tcccaaatgg gtatatgcct tgccaagtaa ggtgaccgga
      181 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      241 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      301 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      361 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
      421 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gacctcgagg gggggcccgg
      481 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      541 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      601 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      661 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      721 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctt
//
