LOCUS       sequence-16581-e        1375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD22_11
FEATURES             Location/Qualifiers
     source          1..1375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(248..270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gnngnggtcg actctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga
       61 atgatactaa cataacatag aacattttca ggaggaccct tgcttggagg gtaccgagct
      121 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtgggtgaa gaccagaaac
      181 agcacctcga actgagccgc gatattgccc agcgtttcaa cgcgctgtat ggcgagatcg
      241 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      301 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      361 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
      421 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
      481 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
      541 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
      601 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
      661 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
      721 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
      781 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
      841 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
      901 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggangctgaa gttcagatgt
      961 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
     1021 tcgccagcgg cacngcgcct tttcgggggg gaaattatcg atgancgngg nggttatgcc
     1081 gatcgcgtca cactacgtct gaacgtcaaa aacccgaant gggggaggnc naaatcccna
     1141 atcttttngn gggggggtna antgcncccc cccangnccc cttatnnaaa naaaacntgn
     1201 nagtcgtttc cncaggggnn atnaaanggt tgntttgtta aagnaaacct ttnttatnna
     1261 ggntnaccnn cnanaatctc ntnttggnng gntgggnnaa aaaaaggggg natccttttt
     1321 aaaaaaaaat naaccggggg tggttnncna nccccgggac cgggnccccc ccttt
//
