LOCUS       sequence-165811-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(210..243)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     247..267
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        562..865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacgg
       61 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
      121 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
      181 tacattttac atgataggct tggatttcta taacttcgta tagcatacat tatacgaagt
      241 tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta
      301 taagtatccc ttggagaacc accttgttgg aaagtcccca ggctccccag cgtttaagag
      361 ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag
      421 tggcaccgag tcggtgcttt ttttctcgag tactaggatc cattaggcgg ccgcgtggat
      481 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
      541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccat
//
