LOCUS       sequence-165816-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(208..241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     245..265
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        348..423
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        548..851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1006..1026
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggcccgctct agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac
       61 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
      121 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
      181 cattttacat gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta
      241 taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
      301 agtatccctt ggagaaccac cttgttggtt actccctatc agtgatagtt atagtactct
      361 ggaaacagaa tctactataa caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg
      421 agattttttt ctcgagtact aggatccatt aggcggccgc gtggataacc gtattaccgc
      481 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      541 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      601 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      841 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      901 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      961 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1021 ggcgtgtacg gtgggaggtc tatataagca
//
