LOCUS       sequence-165825-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eGFP reporter donor containing single SA cutting site for NHEJ 
            knock-in.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: single cut NH-donor
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..96
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    97..321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     310..327
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(404..425)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     753..772
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg taccgggccc cccctcgaca ttaagggttc
       61 cgataacttc gtataatgta tgctatacga agttatccat agagcccacc gcatccccag
      121 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
      181 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
      241 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagata
      301 gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctagatcat cgatgcattt
      361 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      421 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
      481 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      541 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
      601 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      661 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      721 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      781 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      841 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
      901 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      961 a
//
