LOCUS       sequence-165830-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replicating base vector for constructing cpf1/CRISPR editing 
            plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Ungerer J, Pakrasi HB
  TITLE     Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse 
            Species of Cyanobacteria.
  JOURNAL   Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681.
  PUBMED    28000776
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-21"; volume: "6:39681. doi"; pages: " 
            10.1038/srep39681"
COMMENT     Sequence Label: pSL2680
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..40
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(108..404)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     254..276
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     268..285
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     269..285
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     295..314
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        295..313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(361..381)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(361..379)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(400..416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(413..435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(448..454,455..472,473..478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    493..514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aattcttgac agctagctca gtcctaggta taatgctagc gctgatttag gcaaaaacgg
       61 gtctaagaac tttaaataat ttctactgtt gtagatgaga gcaggtgtca gccgctacag
      121 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      181 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      241 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      301 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcctcca gcttttgttc
      361 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      541 ccacccacct gcaaaagtct aagaacttta aataatttct actgttgtag atgctactat
      601 tcctgtgcct tcagataatt cagtctaaga actttaaata atttctactg ttgtagatgt
      661 ctagagcctt ttgtattagt agccggtcta agaactttaa ataatttcta ctgttgtaga
      721 ttagcgattt atgaaggtca tttttttgtc tagctttaat gcggtagttg gtaccgtcga
      781 ctctaga
//
