LOCUS       sequence-165831-        3951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replicating base vector for constructing cpf1/CRISPR editing 
            plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ungerer J, Pakrasi HB
  TITLE     Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse 
            Species of Cyanobacteria.
  JOURNAL   Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681.
  PUBMED    28000776
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-21"; volume: "6:39681. doi"; pages: " 
            10.1038/srep39681"
COMMENT     Sequence Label: pSL2680
FEATURES             Location/Qualifiers
     source          1..3951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(2..7,8..25,26..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ctttacactt tatgcttccg gctcgtatgt taggaggtct ttatcatgtc aatttatcaa
       61 gaatttgtta ataaatatag tttaagtaaa actctaagat ttgagttaat cccacagggt
      121 aaaacacttg aaaacataaa agcaagaggt ttgattttag atgatgagaa aagagctaaa
      181 gactacaaaa aggctaaaca aataattgat aaatatcatc agttttttat agaggagata
      241 ttaagttcgg tttgtattag cgaagattta ttacaaaact attctgatgt ttattttaaa
      301 cttaaaaaga gtgatgatga taatctacaa aaagatttta aaagtgcaaa agatacgata
      361 aagaaacaaa tatctgaata tataaaggac tcagagaaat ttaagaattt gtttaatcaa
      421 aaccttatcg atgctaaaaa agggcaagag tcagatttaa ttctatggct aaagcaatct
      481 aaggataatg gtatagaact atttaaagcc aatagtgata tcacagatat agatgaggcg
      541 ttagaaataa tcaaatcttt taaaggttgg acaacttatt ttaagggttt tcatgaaaat
      601 agaaaaaatg tttatagtag caatgatatt cctacatcta ttatttatag gatagtagat
      661 gataatttgc ctaaatttct agaaaataaa gctaagtatg agagtttaaa agacaaagct
      721 ccagaagcta taaactatga acaaattaaa aaagatttgg cagaagagct aacctttgat
      781 attgactaca aaacatctga agttaatcaa agagtttttt cacttgatga agtttttgag
      841 atagcaaact ttaataatta tctaaatcaa agtggtatta ctaaatttaa tactattatt
      901 ggtggtaaat ttgtaaatgg tgaaaataca aagagaaaag gtataaatga atatataaat
      961 ctatactcac agcaaataaa tgataaaaca ctcaaaaaat ataaaatgag tgttttattt
     1021 aagcaaattt taagtgatac agaatctaaa tcttttgtaa ttgataagtt agaagatgat
     1081 agtgatgtag ttacaacgat gcaaagtttt tatgagcaaa tagcagcttt taaaacagta
     1141 gaagaaaaat ctattaaaga aacactatct ttattatttg atgatttaaa agctcaaaaa
     1201 cttgatttga gtaaaattta ttttaaaaat gataaatctc ttactgatct atcacaacaa
     1261 gtttttgatg attatagtgt tattggtaca gcggtactag aatatataac tcaacaaata
     1321 gcacctaaaa atcttgataa ccctagtaag aaagagcaag aattaatagc caaaaaaact
     1381 gaaaaagcaa aatacttatc tctagaaact ataaagcttg ccttagaaga atttaataag
     1441 catagagata tagataaaca gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt
     1501 ccgatgatat ttgatgaaat agctcaaaac aaagacaatt tggcacagat atctatcaaa
     1561 tatcaaaatc aaggtaaaaa agacctactt caagctagtg cggaagatga tgttaaagct
     1621 atcaaggatc ttttagatca aactaataat ctcttacata aactaaaaat atttcatatt
     1681 agtcagtcag aagataaggc aaatatttta gacaaggatg agcattttta tctagtattt
     1741 gaggagtgct actttgagct agcgaatata gtgcctcttt ataacaaaat tagaaactat
     1801 ataactcaaa agccatatag tgatgagaaa tttaagctca attttgagaa ctcgactttg
     1861 gctaatggtt gggataaaaa taaagagcct gacaatacgg caattttatt tatcaaagat
     1921 gataaatatt atctgggtgt gatgaataag aaaaataaca aaatatttga tgataaagct
     1981 atcaaagaaa ataaaggcga gggttataaa aaaattgttt ataaactttt acctggcgca
     2041 aataaaatgt tacctaaggt tttcttttct gctaaatcta taaaatttta taatcctagt
     2101 gaagatatac ttagaataag aaatcattcc acacatacaa aaaatggtag tcctcaaaaa
     2161 ggatatgaaa aatttgagtt taatattgaa gattgccgaa aatttataga tttttataaa
     2221 cagtctataa gtaagcatcc ggagtggaaa gattttggat ttagattttc tgatactcaa
     2281 agatataatt ctatagatga attttataga gaagttgaaa atcaaggcta caaactaact
     2341 tttgaaaata tatcagagag ctatattgat agcgtagtta atcagggtaa attgtaccta
     2401 ttccaaatct ataataaaga tttttcagct tatagcaaag ggcgaccaaa tctacatact
     2461 ttatattgga aagcgctgtt tgatgagaga aatcttcaag atgtggttta taagctaaat
     2521 ggtgaggcag agctttttta tcgtaaacaa tcaataccta aaaaaatcac tcacccagct
     2581 aaagaggcaa tagctaataa aaacaaagat aatcctaaaa aagagagtgt ttttgaatat
     2641 gatttaatca aagataaacg ctttactgaa gataagtttt tctttcactg tcctattaca
     2701 atcaatttta aatctagtgg agctaataag tttaatgatg aaatcaattt attgctaaaa
     2761 gaaaaagcaa atgatgttca tatattaagt atagatagag gtgaaagaca tttagcttac
     2821 tatactttgg tagatggtaa aggcaatatc atcaaacaag atactttcaa catcattggt
     2881 aatgatagaa tgaaaacaaa ctaccatgat aagcttgctg caatagagaa agatagggat
     2941 tcagctagga aagactggaa aaagataaat aacatcaaag agatgaaaga gggctatcta
     3001 tctcaggtag ttcatgaaat agctaagcta gttatagagt ataatgctat tgtggttttt
     3061 gaggatttaa attttggatt taaaagaggg cgtttcaagg tagagaagca ggtctatcaa
     3121 aagttagaaa aaatgctaat tgagaaacta aactatctag ttttcaaaga taatgagttt
     3181 gataaaactg ggggagtgct tagagcttat cagctaacag caccttttga gacttttaaa
     3241 aagatgggta aacaaacagg tattatctac tatgtaccag ctggttttac ttcaaaaatt
     3301 tgtcctgtaa ctggttttgt aaatcagtta tatcctaagt atgaaagtgt cagcaaatct
     3361 caagagttct ttagtaagtt tgacaagatt tgttataacc ttgataaggg ctattttgag
     3421 tttagttttg attataaaaa ctttggtgac aaggctgcca aaggcaagtg gactatagct
     3481 agctttggga gtagattgat taactttaga aattcagata aaaatcataa ttgggatact
     3541 cgagaagttt atccaactaa agagttggag aaattgctaa aagattattc tatcgaatat
     3601 gggcatggcg aatgtatcaa agcagctatt tgcggtgaga gcgacaaaaa gttttttgct
     3661 aagctaacta gtgtcctaaa tactatctta caaatgcgta actcaaaaac aggtactgag
     3721 ttagattatc taatttcacc agtagcagat gtaaatggca atttctttga ttcgcgacag
     3781 gcgccaaaaa atatgcctca agatgctgat gccaatggtg cttatcatat tgggctaaaa
     3841 ggtctgatgc tactaggtag gatcaaaaat aatcaagagg gcaaaaaact caatttggtt
     3901 atcaaaaatg aagagtattt tgagttcgtg cagaatagga ataactaaga a
//
