LOCUS       sequence-165832-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eGFP reporter donor containing double SA cutting sites for NHEJ 
            knock-in.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: double cut NH-donor
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    128..352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     341..358
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(435..456)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaagggaaca aaagctggta ccgggccccc cctcgaggtt agagagttaa cccggtgatg
       61 cggcactcga tctcgtcgac attaagggtt ccgataactt cgtataatgt atgctatacg
      121 aagttatcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
      181 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
      241 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
      301 gaaggcacgg gggaggggca aacaacagat agctggcaac tagaaggcac agtcgaggct
      361 gatcagcgag ctctagatca tcgatgcatt tacttgtaca gctcgtccat gccgagagtg
      421 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      481 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      541 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      601 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      661 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      721 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      781 c
//
