LOCUS       sequence-165837-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA targeting SA site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: Sg-A
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(266..514)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(324..343)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(494..514)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcat
      121 atgtagagag gtacctcgag cggcccaagc ttaaaaaaag caccgactcg gtgccacttt
      181 ttcaagttga taacggacta gccttattta aacttgctat gctgtttcca gcatagctct
      241 taaacgtgat gcggcactcg atctcggtgt ttcgtccttt ccacaagata tataaagcca
      301 agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt
      361 ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata
      421 tctttgtgtt tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat
      481 gcaaatatga aggaatcatg ggaaataggc cctcttcctg cccgaccttg gtaccggatc
      541 cagtcgactg aattggttcc tttaaagcct gcttttttgt acagctagcg acgtcaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      901 gccccgaaga acgttttcca atgatgagca cttt
//
