LOCUS       sequence-165838-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(208..241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     245..265
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        559..862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcccgctct agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac
       61 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
      121 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
      181 cattttacat gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta
      241 taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
      301 agtatccctt ggagaaccac cttgttggtg gcatgctcac ttcaggtggt ttaagagcta
      361 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
      421 caccgagtcg gtgctttttt tctcgagtac taggatccat taggcggccg cgtggataac
      481 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
      541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
//
