LOCUS       sequence-165842-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Sg-1 sgRNA targeting GAPDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: sgRNA 1 GAPDH
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(161..236)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(257..505)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(315..334)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(485..505)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        612..716
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(935..954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtca
      121 tatgtagaga ggtacctcga gcggcccaag cttaaaaaaa gcaccgactc ggtgccactt
      181 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaaccagc
      241 agtgagggtc tctctcggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga
      301 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
      361 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
      421 ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg
      481 aaggaatcat gggaaatagg ccctcttcct gcccgacctt ggtaccggat ccagtcgact
      541 gaattggttc ctttaaagcc tgcttttttg tacagctagc gacgtcaggt ggcacttttc
      601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      961 ttgac
//
