LOCUS       sequence-165845-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(210..243)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     247..267
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        561..864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacgg
       61 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
      121 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
      181 tacattttac atgataggct tggatttcta taacttcgta tagcatacat tatacgaagt
      241 tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta
      301 taagtatccc ttggagaacc accttgttgg aagcctcgct ggggaacgcc gtttaagagc
      361 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcggc cgcgtggata
      481 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
      541 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      601 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      661 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      721 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      781 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      841 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      901 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      961 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgac
//
