LOCUS       sequence-165848-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Sg-2 sgRNA targeting GAPDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: sgRNA 2 GAPDH
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(263..511)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(321..340)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(491..511)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        618..722
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(941..960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcatatg
      121 tagagaggta cctcgagcgg cccaagctta aaaaaagcac cgactcggtg ccactttttc
      181 aagttgataa cggactagcc ttatttaaac ttgctatgct gtttccagca tagctcttaa
      241 accttgtgct cttgctgggg ctggtgtttc gtcctttcca caagatatat aaagccaaga
      301 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
      361 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
      421 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
      481 aatatgaagg aatcatggga aataggccct cttcctgccc gaccttggta ccggatccag
      541 tcgactgaat tggttccttt aaagcctgct tttttgtaca gctagcgacg tcaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      961 cccgtattga cgccggg
//
