LOCUS       sequence-165850-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(208..241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     245..265
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        351..426
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        551..854
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcccgctct agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac
       61 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
      121 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
      181 cattttacat gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta
      241 taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
      301 agtatccctt ggagaaccac cttgttggca gacgcgagga aggagggcgc gttatagtac
      361 tctggaaaca gaatctacta taacaaggca aaatgccgtg tttatctcgt caacttgttg
      421 gcgagatttt tttctcgagt actaggatcc attaggcggc cgcgtggata accgtattac
      481 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      541 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      601 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      661 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      721 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      781 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      841 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      901 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      961 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgac
//
