LOCUS       sequence-165861-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta19) ETFalpha wt without
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
       61 gatatacata tgcagagtac cctggtaata gctgagcatg caaatgattc cctagcaccc
      121 attactttaa ataccattac tgcagccaca cgccttggag gtgaagtgtc ctgcttagta
      181 gctggaacca aatgtgacaa ggtggcacaa gatctctgta aagtagcagg catagcaaaa
      241 gttctggtgg ctcagcatga tgtgtacaaa ggcctacttc cagaggaact gacaccattg
      301 attttggcaa ctcagaagca gttcaattac acacacatct gtgctggagc atctgccttc
      361 ggaaagaacc ttttgcccag agtagcagcc aaacttgagg ttgccccgat ttctgacatc
      421 attgcaatca agtcacctga cacatttgtg agaactattt atgcaggaaa tgctctatgt
      481 acagtgaagt gtgatgagaa agtgaaagtg ttttctgtcc gtggaacatc ctttgatgct
      541 gcagcaacaa gtggcggtag tgccagttca gaaaaggcat caagtacttc accagtggaa
      601 atatcagagt ggcttgacca gaaattaaca aaaagtgatc gaccagagct aacaggtgcc
      661 aaagtggtgg tatctggtgg tcgaggcttg aagagtggag agaactttaa gttgttatat
      721 gacttggcag atcaactaca tgctgcagtt ggtgcttccc gtgctgctgt tgatgctggc
      781 tttgttccca atgacatgca agttggacag acgggaaaaa tagtagcacc agaactttat
      841 attgctgttg gaatatctgg agccatccaa catttagctg ggatgaaaga cagcaagaca
      901 att
//
