LOCUS       sequence-165863-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta19) ETFalpha wt without
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..58)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctgcgctagt agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac
       61 cagactcgag tcattttttc ttcaatatct cagtcatttc aggaactacc ttaaataaat
      121 ctgcaactat tccataatct gccacttgga aaattggagc ttctgggtct ttattaattg
      181 ccacaattgt cttgctgtct ttcatcccag ctaaatgttg gatggctcca gatattccaa
      241 cagcaatata aagttctggt gctactattt ttcccgtctg tccaacttgc atgtcattgg
      301 gaacaaagcc agcatcaaca gcagcacggg aagcaccaac tgcagcatgt agttgatctg
      361 ccaagtcata taacaactta aagttctctc cactcttcaa gcctcgacca ccagatacca
      421 ccactttggc acctgttagc tctggtcgat cactttttgt taatttctgg tcaagccact
      481 ctgatatttc cactggtgaa gtacttgatg ccttttctga actggcacta ccgccacttg
      541 ttgctgcagc atcaaaggat gttccacgga cagaaaacac tttcactttc tcatcacact
      601 tcactgtaca tagagcattt cctgcataaa tagttctcac aaatgtgtca ggtgacttga
      661 ttgcaatgat gtcagaaatc ggggcaacct caagtttggc tgctactctg ggcaaaaggt
      721 tctttccgaa ggcagatgct ccagcacaga tgtgtgtgta attgaactgc ttctgagttg
      781 ccaaaatcaa tggtgtcagt tcctctggaa gtaggccttt gtacacatca tgctgagcca
      841 ccagaacttt tgctatgcct gctacttt
//
