LOCUS       sequence-165866-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta25) MCAD wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF711-pET28a-Hs-delta25-MCAD-NHis wt
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg aaagccaatc
      121 gacaacgtga accaggatta ggatttagtt ttgagttcac cgaacagcag aaagaatttc
      181 aagctactgc tcgtaaattt gccagagagg aaatcatccc agtggctgca gaatatgata
      241 aaactggtga atatccagtc cccctaatta gaagagcctg ggaacttggt ttaatgaaca
      301 cacacattcc agagaactgt ggaggtcttg gacttggaac ttttgatgct tgtttaatta
      361 gtgaagaatt ggcttatgga tgtacagggg ttcagactgc tattgaagga aattctttgg
      421 ggcaaatgcc tattattatt gctggaaatg atcaacaaaa gaagaagtat ttggggagaa
      481 tgactgagga gccattgatg tgtgcttatt gtgtaacaga acctggagca ggctctgatg
      541 tagctggtat aaagaccaaa gcagaaaaga aaggagatga gtatattatt aatggtcaga
      601 agatgtggat aaccaacgga ggaaaagcta attggtattt tttattggca cgttctgatc
      661 cagatcctaa agctcctgct aataaagcct ttactggatt cattgtggaa gcagataccc
      721 caggaattca gattgggaga aaggaattaa acatgggcca gcgatgttca gatactagag
      781 gaattgtctt cgaagatgtg aaagtgccta aagaaaatgt tttaattggt gacggagctg
      841 gtttcaaagt tgcaatggga gcttttgata aaaccagacc tgtagtagct gctggtgctg
      901 ttggattagc acaaagagct ttggatgaag ctaccaagta tgccctggaa aggaaaactt
      961 tcggaaagct acttgtagag caccaagcaa tatcatttat gctggctgaa atggcaatga
     1021 aagttgaact agctagaatg a
//
