LOCUS       sequence-165867-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta25) MCAD wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF711-pET28a-Hs-delta25-MCAD-NHis wt
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcttaatt tttgtacttg tcaatgtgtt cacgggctac aataagtctt tgaatttgtg
      121 aagtaccttc ataaatctga tagattttgg catccctcat tagtttttct acaggatatt
      181 ctgtattaaa tccattgcct ccaagtatct gcacagcatc agtagctaac tgatttgcaa
      241 tatctccagc aaatgccttt gcaatagaag cataataggt atttcgacga ccagaatcaa
      301 cctcccaagc tgctctctgg taactcattc tagctagttc aactttcatt gccatttcag
      361 ccagcataaa tgatattgct tggtgctcta caagtagctt tccgaaagtt ttcctttcca
      421 gggcatactt ggtagcttca tccaaagctc tttgtgctaa tccaacagca ccagcagcta
      481 ctacaggtct ggttttatca aaagctccca ttgcaacttt gaaaccagct ccgtcaccaa
      541 ttaaaacatt ttctttaggc actttcacat cttcgaagac aattcctcta gtatctgaac
      601 atcgctggcc catgtttaat tcctttctcc caatctgaat tcctggggta tctgcttcca
      661 caatgaatcc agtaaaggct ttattagcag gagctttagg atctggatca gaacgtgcca
      721 ataaaaaata ccaattagct tttcctccgt tggttatcca catcttctga ccattaataa
      781 tatactcatc tcctttcttt tctgctttgg tctttatacc agctacatca gagcctgctc
      841 caggttctgt tacacaataa gcacacatca atggctcctc agtcattctc cccaaatact
      901 tcttcttttg t
//
