LOCUS       sequence-165871-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta44) GCDH wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF712-pET28a-Hs-delta44-GCDH-NHis wt
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgcg tcccgagttt gactggcagg
      121 acccgctggt gctggaggag cagctgacca cagatgagat cctcatcagg gacaccttcc
      181 gcacctactg ccaggagaga ctcatgcctc gcatcctgtt ggccaatcgc aacgaagttt
      241 ttcatcggga gatcatttcg gagatggggg agttgggtgt gctgggcccc accatcaaag
      301 gatatggctg tgctggggtt tcgtctgtgg cctatgggct cctggcccga gagctggagc
      361 gggtggacag tggctacagg tcggcgatga gtgtccagtc ctccctcgtc atgcacccta
      421 tctatgccta tggcagcgag gaacagcggc agaagtacct gccccagctg gccaaggggg
      481 agctcctggg ctgcttcggg ctcacagagc ccaacagcgg aagtgacccc agcagcatgg
      541 agaccagagc ccactacaac tcatccaaca agagctacac cctcaatggg accaagacct
      601 ggatcacgaa ctcgcctatg gccgatctgt ttgtagtgtg ggctcggtgt gaagatggct
      661 gcattcgggg cttcctgctg gagaagggga tgcggggtct ctcggccccc aggatccagg
      721 gcaagttctc gctgcgggcc tcagccacag gcatgatcat catggacggt gtggaggtgc
      781 cagaggagaa tgtgctccct ggtgcatcca gcctgggggg tcccttcggc tgcctgaaca
      841 acgcccggta cggcatcgcg tggggcgtgc ttggagcttc ggagttctgc ttgcacacag
      901 cccggcagta cgccctcgac aggatgcagt ttggtgtccc actgg
//
