LOCUS       sequence-165873-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta44) GCDH wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF712-pET28a-Hs-delta44-GCDH-NHis wt
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgctcacttg ctggccgtga acgcctggat tcccgtgata gctctcccaa ggatcagggc
      121 gtgaatgtca tgtgtacctt cgtaggtgtt cacggcctcc aggttcatgg cgtgccggat
      181 cacgtgatac tcgtcagaaa tcccattacc ccccagcatg tctcgggcct ggcgggcgat
      241 gtccagggct ttcccacagt tattcctctt cagcagagaa accatctcgg gggcagcctt
      301 gtcctggtcc ttcaagcggc cgagctgcag gcaggcgtga aggcccaggg taatctcagt
      361 gagcatgtct gccagcttct tctgaatcag ctggttcctg gccagtggga caccaaactg
      421 catcctgtcg agggcgtact gccgggctgt gtgcaagcag aactccgaag ctccaagcac
      481 gccccacgcg atgccgtacc gggcgttgtt caggcagccg aagggacccc ccaggctgga
      541 tgcaccaggg agcacattct cctctggcac ctccacaccg tccatgatga tcatgcctgt
      601 ggctgaggcc cgcagcgaga acttgccctg gatcctgggg gccgagagac cccgcatccc
      661 cttctccagc aggaagcccc gaatgcagcc atcttcacac cgagcccaca ctacaaacag
      721 atcggccata ggcgagttcg tgatccaggt cttggtccca ttgagggtgt agctcttgtt
      781 ggatgagttg tagtgggctc tggtctccat gctgctgggg tcacttccgc tgttgggctc
      841 tgtgagcccg aagcagccca ggagctcccc cttggccagc tggggcaggt acttctgccg
      901 ctgttcctcg ctgccatagg catagatagg gtgcatgacg agggaggact ggacactcat
      961 cgccga
//
