LOCUS       sequence-165874-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transfection of HeLa cell for transient expression of human 
            full-length METTL20-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF713-pEGFP-N1-Hs-FL-METTL20 wt
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
      301 ggcccgcgga ggctgaaaac cccacactgt gcttgttgtc agtccactgt tttcctgcct
      361 agtagactcc aaaagtgaat attctaccac tttgtgcagg tgatgctgaa tgctgtgtcc
      421 actgaactgg ggccgcccag ggtcaccaat cagtactcga gttctatagg tccagaagca
      481 cttcttcagc cactgatgaa gactatctgc aaggtcttca tcataaaaca tatcgccaag
      541 aacaacaagg tcccacttat cttgttccaa attcaaaatg ttttggatta aaataggaaa
      601 aggattcagt ctgttcaatt cacaatttag tgtaatagcc attcctgcaa tagggtctat
      661 gtcattggcc aagatccttg atgccccact catcttagca gcaatagctg tagctccaca
      721 tccactccca agatctaata cagattttcc tctgacaaca tcaggattat ccaaaagata
      781 cctagacagg gcttggcctc ctggccagta gattgcccag taaggatcac tgtggggcca
      841 caggtcagct ctctcccacc agaatttgca tctgggggtc aaaagccgca actggatttc
      901 aggggtga
//
