LOCUS       sequence-165876-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transfection of HeLa cell for transient expression of human 
            full-length METTL20-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF713-pEGFP-N1-Hs-FL-METTL20 wt
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      873..882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(924..945)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcatggcttt gagtctaggt tggaaagcac acaggaacca ctgtggtctc ctcttgcagg
      121 ctctgcgaag cagtggtctt ctcttgtttc cctgtggcca gtgtccctgg agaggagctg
      181 gaagcttttt ggaccctgag ataaaggctt tcctggagga gaacactgaa gtcaccagca
      241 gtggtagcct cacccctgaa atccagttgc ggcttttgac ccccagatgc aaattctggt
      301 gggagagagc tgacctgtgg ccccacagtg atccttactg ggcaatctac tggccaggag
      361 gccaagccct gtctaggtat cttttggata atcctgatgt tgtcagagga aaatctgtat
      421 tagatcttgg gagtggatgt ggagctacag ctattgctgc taagatgagt ggggcatcaa
      481 ggatcttggc caatgacata gaccctattg caggaatggc tattacacta aattgtgaat
      541 tgaacagact gaatcctttt cctattttaa tccaaaacat tttgaatttg gaacaagata
      601 agtgggacct tgttgttctt ggcgatatgt tttatgatga agaccttgca gatagtcttc
      661 atcagtggct gaagaagtgc ttctggacct atagaactcg agtactgatt ggtgaccctg
      721 ggcggcccca gttcagtgga cacagcattc agcatcacct gcacaaagtg gtagaatatt
      781 cacttttgga gtctactagg caggaaaaca gtggactgac aacaagcaca gtgtggggtt
      841 ttcagcctcc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
      901 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      961 tca
//
