LOCUS       sequence-165879-       14298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dosage control and tracing of reprogramming episomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Schmitt CE, Morales BM, Schmitz EMH, Hawkins JS, Lizama CO, Zape JP,
            Hsiao EC, Zovein AC
  TITLE     Fluorescent tagged episomals for stoichiometric induced pluripotent 
            stem cell reprogramming.
  JOURNAL   Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 
            10.1186/s13287-017-0581-7.
  PUBMED    28583172
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther."; date: "2017-06-5"; volume: "8(1):132. doi"; pages: " 
            10.1186/s13287-017-0581-7"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
FEATURES             Location/Qualifiers
     source          1..14298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2866..3454
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2919..2939)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3337..3348)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3544..3563)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3609..3664
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3663..3682)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4025..4041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4025..4041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4038..4060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4049..4065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4073..4079,4080..4097,4098..4103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4118..4139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4228..4261
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4701..4925
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4932..5528)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5015..5035)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(5532..5585)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5592..6299)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5867..5886)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6136..6154
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6391..6415)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6412..6615)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6441..6461)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6616..6919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             7339..9264
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    9432..9465
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      9652..11441
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        11912..12225
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     12144..12164
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(12376..12393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12547..13135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12627..12646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13306..14097,14098..14166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13929..13948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14167..14271)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
     1741 ccatggcggg acacctggct tcggatttcg ccttctcgcc ccctccaggt ggtggaggtg
     1801 atgggccagg ggggccggag ccgggctggg ttgatcctcg gacctggcta agcttccaag
     1861 gccctcctgg agggccagga atcgggccgg gggttgggcc aggctctgag gtgtggggga
     1921 ttcccccatg ccccccgccg tatgagttct gtggggggat ggcgtactgt gggccccagg
     1981 ttggagtggg gctagtgccc caaggcggct tggagacctc tcagcctgag ggcgaagcag
     2041 gagtcggggt ggagagcaac tccgatgggg cctccccgga gccctgcacc gtcacccctg
     2101 gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc ggaggagtcc caggacatca
     2161 aagctctgca gaaagaactc gagcaatttg ccaagctcct gaagcagaag aggatcaccc
     2221 tgggatatac acaggccgat gtggggctca ccctgggggt tctatttggg aaggtattca
     2281 gccaaacgac catctgccgc tttgaggctc tgcagcttag cttcaagaac atgtgtaagc
     2341 tgcggccctt gctgcagaag tgggtggagg aagctgacaa caatgaaaat cttcaggaga
     2401 tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa gcgaaccagt atcgagaacc
     2461 gagtgagagg caacctggag aatttgttcc tgcagtgccc gaaacccaca ctgcagcaga
     2521 tcagccacat cgcccagcag cttgggctcg agaaggatgt ggtccgagtg tggttctgta
     2581 accggcgcca gaagggcaag cgatcaagca gcgactatgc acaacgagag gattttgagg
     2641 ctgctgggtc tcctttctca gggggaccag tgtcctttcc tctggcccca gggccccatt
     2701 ttggtacccc aggctatggg agccctcact tcactgcact gtactcctcg gtccctttcc
     2761 ctgaggggga agcctttccc cctgtctctg tcaccactct gggctctccc atgcattcaa
     2821 actgaggtaa gggcgaattc aagcttcggg gactagtcat atgataatca acctctggat
     2881 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2941 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3001 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3061 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3121 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3181 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3241 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3301 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3361 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3421 acgagtcgga tctccctttg ggccgcctcc ccgcatcggt aaattcactc ctcaggtgca
     3481 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     3541 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     3601 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3661 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     3721 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     3781 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     3841 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     3901 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     3961 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     4021 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4081 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4141 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatctcaa
     4201 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
     4261 ttaggtctga agaggagttt acgtccagcc aagcttagga tcaattctca tgtttgacag
     4321 cttatcatcg atggccggcc ggcgcccttg ctcaccatga ccggtggatc ccgggccctg
     4381 gtgagcctgg cctctcctta cctcaggtcc gtgggaggct gggggctggg ctgccgccgc
     4441 cgctgccacc tgtccacact gtttatctgg ctgagtggtt tctggcatgc cccgcagagg
     4501 tgactgggac ctctgaggag ccgctgctgc cgcctcagtt cggaatcgct gcatcccagg
     4561 ctggggatag acatggaaag ggtgcacctg ccggttctca gcgccttcat ggagcacagg
     4621 aatgggaatg tagcctggtc ggagctgggg gtacacaggg tggccttccc tagcaggcgg
     4681 cagcctagag ccctcccggg ccatagagcc caccgcatcc ccagcatgcc tgctattgtc
     4741 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
     4801 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
     4861 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
     4921 cacaggaatt cttaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     4981 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     5041 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     5101 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
     5161 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     5221 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     5281 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     5341 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     5401 tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     5461 gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     5521 tactcggttc gagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg
     5581 ccctcccggt gcttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     5641 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     5701 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     5761 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     5821 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     5881 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     5941 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     6001 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     6061 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     6121 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     6181 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     6241 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatc
     6301 tcgagatctg agtccggtag cgctagcgat cctctagtag agtcggtgtc ttctatggag
     6361 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
     6421 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     6481 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     6541 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     6601 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     6661 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     6721 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     6781 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     6841 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     6901 taccgtaagt tatgtaacgc ggaactccca agcttgcgat cgcatcgata agctgatcct
     6961 cacaggccgc acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg
     7021 aagaggaaga ggaggggtcc cgagaatccc catccctacc gtccagcaaa aagggggacg
     7081 aggaatttga ggcctggctt gaggctcagg acgcaaatct tgaggatgtt cagcgggagt
     7141 tttccgggct gcgagtaatt ggtgatgagg acgaggatgg ttcggaggat ggggaatttt
     7201 cagacctgga tctgtctgac agcgaccatg aaggggatga gggtgggggg gctgttggag
     7261 ggggcaggag tctgcactcc ctgtattcac tgagcgtcgt ctaataaaga tgtctattga
     7321 tctcttttag tgtgaatcat gtctgacgag gggccaggta caggacctgg aaatggccta
     7381 ggagagaagg gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga
     7441 gggggtgata accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca
     7501 ggagccccgg gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa
     7561 aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga
     7621 gcgggagggg caggagcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
     7681 gcaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga
     7741 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca
     7801 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
     7861 gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga
     7921 ggggcaggag caggaggagg ggcaggagca ggaggggcag gaggggcagg aggggcagga
     7981 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggaggg
     8041 gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggggcagga
     8101 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg
     8161 gcaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg ggcaggaggg
     8221 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     8281 ggaggggcag gagcaggagg ggcaggagca ggaggtggag gccggggtcg aggaggcagt
     8341 ggaggccggg gtcgaggagg tagtggaggc cggggtcgag gaggtagtgg aggccgccgg
     8401 ggtagaggac gtgaaagagc caggggggga agtcgtgaaa gagccagggg gagaggtcgt
     8461 ggacgtggag aaaagaggcc caggagtccc agtagtcagt catcatcatc cgggtctcca
     8521 ccgcgcaggc cccctccagg tagaaggcca tttttccacc ctgtagggga agccgattat
     8581 tttgaatacc accaagaagg tggcccagat ggtgagcctg acgtgccccc gggagcgata
     8641 gagcagggcc ccgcagatga cccaggagaa ggcccaagca ctggaccccg gggtcagggt
     8701 gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc atcgtggtca aggaggttcc
     8761 aacccgaaat ttgagaacat tgcagaaggt ttaagagctc tcctggctag gagtcacgta
     8821 gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag
     8881 acctcccttt acaacctaag gcgaggaact gcccttgcta ttccacaatg tcgtcttaca
     8941 ccattgagtc gtctcccctt tggaatggcc cctggacccg gcccacaacc tggcccgcta
     9001 agggagtcca ttgtctgtta tttcatggtc tttttacaaa ctcatatatt tgctgaggtt
     9061 ttgaaggatg cgattaagga ccttgttatg acaaagcccg ctcctacctg caatatcagg
     9121 gtgactgtgt gcagctttga cgatggagta gatttgcctc cctggtttcc acctatggtg
     9181 gaaggggctg ccgcggaggg tgatgacgga gatgacggag atgaaggagg tgatggagat
     9241 gagggtgagg aagggcagga gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa
     9301 agccgtgtat tcccccgcac taaagaataa atccccagta gacatcatgc gtgctgttgg
     9361 tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc ccaacatggg gcaattgccg
     9421 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgaaga
     9481 ggttcactag cggatctcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct
     9541 caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc
     9601 gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag
     9661 gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact
     9721 cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac
     9781 ccggatacag attaggatag catatactac ccagatatag attaggatag catatgctac
     9841 ccagatatag attaggatag cctatgctac ccagatataa attaggatag catatactac
     9901 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     9961 ccagatatag attaggatag catatgctac ccagatatag attaggatag catatgctat
    10021 ccagatattt gggtagtata tgctacccag atataaatta ggatagcata tactacccta
    10081 atctctatta ggatagcata tgctacccgg atacagatta ggatagcata tactacccag
    10141 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
    10201 atataaatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
    10261 atatagatta ggatagccta tgctacccag atatagatta ggatagcata tgctatccag
    10321 atatttgggt agtatatgct acccatggca acattagccc accgtgctct cagcgacctc
    10381 gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt
    10441 cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta tgcaggtatt
    10501 ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg ttagcggggt
    10561 tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg
    10621 ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt
    10681 ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga gacaaccagt
    10741 ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga gtgtaacaac
    10801 atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag tatcatattg
    10861 cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat ccagtcttta
    10921 cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt ttcattccta
    10981 cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata gcacaatgcc
    11041 accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc ttgttttcga
    11101 gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc taaacgaagg
    11161 agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg atcttcagcc
    11221 actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc atgtaaataa
    11281 aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt ttactaaccc
    11341 taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag ggttagtctg
    11401 gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa caagggggcc
    11461 ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct acacaggccc
    11521 ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga ggtacatgtc
    11581 ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat gttgtgttgc
    11641 agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg caaatgtgca
    11701 catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc
    11761 gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca
    11821 tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg
    11881 gggcatcgat cctctagagt cgacgctagc ggatccgacg ccgccatctc taggcccgcg
    11941 ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc cggttaattt
    12001 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
    12061 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa gagatacaaa
    12121 tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact gtaaagtaat
    12181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttgggc cgactccagt
    12241 ggtaatctac ttcaagagag tagattacca ctggagtctt ttttggaatt cctgcagccc
    12301 gggggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    12361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    12421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    12481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    12541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    13261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    13321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    13381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    13441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    13501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    13561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    13621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    13681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    14221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    14281 ccgaaaagtg ccacctgg
//
