LOCUS       sequence-165881-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human full-length METTL20 wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF714-pET28a-Hs-FL-METTL20-NHis wt
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(856..873)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(883..900)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgctcaaggc tgaaaacccc acactgtgct tgttgtcagt ccactgtttt cctgcctagt
      121 agactccaaa agtgaatatt ctaccacttt gtgcaggtga tgctgaatgc tgtgtccact
      181 gaactggggc cgcccagggt caccaatcag tactcgagtt ctataggtcc agaagcactt
      241 cttcagccac tgatgaagac tatctgcaag gtcttcatca taaaacatat cgccaagaac
      301 aacaaggtcc cacttatctt gttccaaatt caaaatgttt tggattaaaa taggaaaagg
      361 attcagtctg ttcaattcac aatttagtgt aatagccatt cctgcaatag ggtctatgtc
      421 attggccaag atccttgatg ccccactcat cttagcagca atagctgtag ctccacatcc
      481 actcccaaga tctaatacag attttcctct gacaacatca ggattatcca aaagatacct
      541 agacagggct tggcctcctg gccagtagat tgcccagtaa ggatcactgt ggggccacag
      601 gtcagctctc tcccaccaga atttgcatct gggggtcaaa agccgcaact ggatttcagg
      661 ggtgaggcta ccactgctgg tgacttcagt gttctcctcc aggaaagcct ttatctcagg
      721 gtccaaaaag cttccagctc ctctccaggg acactggcca cagggaaaca agagaagacc
      781 actgcttcgc agagcctgca agaggagacc acagtggttc ctgtgtgctt tccaacctag
      841 actcaaagcc atatggctgc cgcgcggcac caggccgctg ctgtgatgat gatgatgatg
      901 gctgctgccc atggtatatc tccttcttaa
//
