LOCUS       sequence-165882-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mock vector for generating FLIP targeting vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, 
            Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
  TITLE     One-step generation of conditional and reversible gene knockouts.
  JOURNAL   Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
  PUBMED    28135257
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156."
COMMENT     Sequence Label: pUC118-FLIP-Puro
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    107..140
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(199..218)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     592..615
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accaataggc agagagagtc agtgcctatc agaaacccaa gagtcttctc tgtctccacg
       61 tgcccagttt ctattggtct ccttaaacct gtcttgtaac ctctagataa cttcgtataa
      121 tgtgtactat acgaagttat cgatggctgt cgaaaggccc ggagatgagg aagaggagaa
      181 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
      241 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
      301 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
      361 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
      421 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
      481 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
      541 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
      601 tgctaaagcg catgctccag actgccttgg gaaa
//
