LOCUS       sequence-165884-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FAM86A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, 
            Loenarz C, Falnes PO
  TITLE     Identification and characterization of a novel evolutionarily 
            conserved lysine-specific methyltransferase targeting eukaryotic 
            translation elongation factor 2 (eEF2).
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 
            10.1074/jbc.M114.601658. Epub 2014 Sep 17.
  PUBMED    25231979
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.601658"; journalName: "J Biol Chem"; date: 
            "2014-10-31- 31"; volume: "289"; issue: "44"; pages: "30499-510"
COMMENT     Sequence Label: pET28a-FAM86A
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatggcgccc gaggagaacg
      121 cggggaccga actcttgctg cagagtttcg agcgccgctt cctggcggca cgcacactgc
      181 gctccttccc ctggcagagc ttagaagcaa agttaagaga ctcatcagat tctgagctgc
      241 tgcgggatat tttgcacaag actgtgaagc atcctgtgtg tgtgaagcac ccgccgtccg
      301 tcaaatatgc ccggtgcttt ctctcagaac tcatcaaaaa gcacgaggct gtccacacag
      361 agcctttgga cgagctgtat gaagcgctgg cggagaccct gatggccaag gagtccaccc
      421 agggccaccg gagctatttg ctgccctcgg gaggctcggt cacactctcc gagagcacgg
      481 ccatcatctc ctacggtacc acaggcctgg tcacatggga cgccgccctc taccttgcag
      541 aatgggccat cgagaacccg gcagtcttca ctaacaggac tgtcctagag cttggcagtg
      601 gtgctggcct cacaggcctg gccatctgca agatgtgccg cccccgggca tacatcttca
      661 gcgactgtca cagccgggtc cttgagcagc tccgagggaa tgtccttctc aatggcctct
      721 cattagaggc agacatcact gccaagttag acagccccag ggtgacagtg gcccagctgg
      781 actgggacgt cgcgacggtc catcagctct ctgccttcca gccagatgtt gtcattgcag
      841 cagatgtgct gtattgccca gaagccatca tgtcgctggt cggcgtcctg cggaggctgg
      901 ctgcctgccg ggagcaccag cgggctcctg aggtctacgt ggcctttacc gtccgcaacc
      961 ca
//
