LOCUS       sequence-165887-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FAM86A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, 
            Loenarz C, Falnes PO
  TITLE     Identification and characterization of a novel evolutionarily 
            conserved lysine-specific methyltransferase targeting eukaryotic 
            translation elongation factor 2 (eEF2).
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 
            10.1074/jbc.M114.601658. Epub 2014 Sep 17.
  PUBMED    25231979
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.601658"; journalName: "J Biol Chem"; date: 
            "2014-10-31- 31"; volume: "289"; issue: "44"; pages: "30499-510"
COMMENT     Sequence Label: pET28a-FAM86A
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcctacagg gtgagattca gcattgccat ctccaagtgc tcttcgtagg gaaacagttt
      121 ctgctcatga cgaggttcca cttcccatct gatcccggcc cggcctagct cggtggtgaa
      181 cagctggcac gtctctgggt tgcggacggt aaaggccacg tagacctcag gagcccgctg
      241 gtgctcccgg caggcagcca gcctccgcag gacgccgacc agcgacatga tggcttctgg
      301 gcaatacagc acatctgctg caatgacaac atctggctgg aaggcagaga gctgatggac
      361 cgtcgcgacg tcccagtcca gctgggccac tgtcaccctg gggctgtcta acttggcagt
      421 gatgtctgcc tctaatgaga ggccattgag aaggacattc cctcggagct gctcaaggac
      481 ccggctgtga cagtcgctga agatgtatgc ccgggggcgg cacatcttgc agatggccag
      541 gcctgtgagg ccagcaccac tgccaagctc taggacagtc ctgttagtga agactgccgg
      601 gttctcgatg gcccattctg caaggtagag ggcggcgtcc catgtgacca ggcctgtggt
      661 accgtaggag atgatggccg tgctctcgga gagtgtgacc gagcctcccg agggcagcaa
      721 atagctccgg tggccctggg tggactcctt ggccatcagg gtctccgcca gcgcttcata
      781 cagctcgtcc aaaggctctg tgtggacagc ctcgtgcttt ttgatgagtt ctgagagaaa
      841 gcaccgggca tatttgacgg acggcgggtg cttcacacac acaggatgct tcacagtctt
      901 gtgcaaaata t
//
