LOCUS       sequence-165889-         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, 
            Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
  TITLE     One-step generation of conditional and reversible gene knockouts.
  JOURNAL   Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
  PUBMED    28135257
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156."
COMMENT     Sequence Label: pUC118-mCtnnb1-FLIP-FlpE-Puro
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(97..130)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    136..169
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(228..247)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct ccacgtgccc
       61 agtttctatt ggtctcctta aacctgtctt gtaaccgaag ttcctatact atctagagaa
      121 taggaacttc tctagataac ttcgtataat gtgtactata cgaagttatc gatggctgtc
      181 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
      241 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
      301 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
      361 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
      421 tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
      481 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
      541 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
//
