LOCUS       sequence-165892-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human VEGFR2 with V5 and tGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Raikwar NS, Shibuya M, Thomas CP
  TITLE     VEGF-A selectively inhibits FLT1 ectodomain shedding independent of 
            receptor activation and receptor endocytosis.
  JOURNAL   Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C214-C224. doi: 
            10.1152/ajpcell.00247.2017. Epub 2018 May 2.
  PUBMED    29719170
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajpcell.00247.2017"; journalName: "Am J Physiol Cell 
            Physiol"; date: "2018-08-1- 1"; volume: "315"; issue: "2"; pages: 
            "C214-C224"
COMMENT     Sequence Label: hKDR
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..40
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..40
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(63..758)
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 agggatgcca cccgggatct gttcaggaaa cagctatgac cgcggccggc cgtttaaact
       61 ctttcttcac cggcatctgc atccggggtc ttgaaggcgt gctggtactc cacgatgccc
      121 agctcggtgt tgctgtgatc ctcctccacg cggcggaagg cgaacatggg gcccccgttc
      181 tgcaggatgc tggggtggat ggcgctcttg aagtgcatgt ggctgtccac cacggagctg
      241 tagtagccgc cgtcgcgcag gctgaaggtg cgggtgaagc tgccatccag atcgttatcg
      301 cccatggggt gcaggtgctc cacggtggcg ttgctgcgga tgatcttgtc ggtgaagatc
      361 acgctgtcct cggggaagcc ggtgcccatc accttgaagt cgccgatcac gcggccggcc
      421 tcgtagcggt agctgaagct cacgtgcagc acgccgccgt cctcgtactt ctcgatgcgg
      481 gtgttggtgt agccgccgtt gttgatggcg tgcaggaagg ggttctcgta gccgctgggg
      541 taggtgccga agtggtagaa gccgtagccc atcacgtggc tcagcaggta ggggctgaag
      601 gtcagggcgc ctttggtgct cttcatcttg ttggtcatgc ggccctgctc gggggtgccc
      661 tctccgccgc ccaccagctc gaactccacg ccgttcaggg tgccggtgat gcggcactcg
      721 atctccatgg cgggcaggcc gctctcgtcg ctctccatct cgagcggccg cgtacgcgta
      781 acaggaggag agctcagtgt ggtccccgag tcaggctgga gaatctgggc tgtgctaccg
      841 gtttgcactc caatctctat cagctttaaa agttctgctt cctcactgga gtacacggtg
//
