LOCUS       sequence-165894-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of FAM86A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, 
            Loenarz C, Falnes PO
  TITLE     Identification and characterization of a novel evolutionarily 
            conserved lysine-specific methyltransferase targeting eukaryotic 
            translation elongation factor 2 (eEF2).
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 
            10.1074/jbc.M114.601658. Epub 2014 Sep 17.
  PUBMED    25231979
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.601658"; journalName: "J Biol Chem"; date: 
            "2014-10-31- 31"; volume: "289"; issue: "44"; pages: "30499-510"
COMMENT     Sequence Label: pcDNA5/FRT/TO_TAP_FAM86A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             195..272
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             291..404
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc catgaagcga cgatggaaaa agaatttcat agccgtctca gcagccaacc
      241 gctttaagaa aatctcatcc tccggggcac ttggaagcgg tagcggtacc atggacgaga
      301 agaccaccgg ctggcggggc ggccacgtgg tggagggcct ggccggcgag ctggagcagc
      361 tgcgggccag gctggagcac caccctcagg gccagcggga gccctccggc ggctgcaagc
      421 tgggcatggc gcccgaggag aacgcgggga ccgaactctt gctgcagagt ttcgagcgcc
      481 gcttcctggc ggcacgcaca ctgcgctcct tcccctggca gagcttagaa gcaaagttaa
      541 gagactcatc agattctgag ctgctgcggg atattttgca caagactgtg aagcatcctg
      601 tgtgtgtgaa gcacccgccg tccgtcaaat atgcccggtg ctttctctca gaactcatca
      661 aaaagcacga ggctgtccac acagagcctt tggacgagct gtatgaagcg ctggcggaga
      721 ccctgatggc caaggagtcc acccagggcc accggagcta tttgctgccc tcgggaggct
      781 cggtcacact ctccgagagc acggccatca tctcctacgg taccacaggc ctggtcacat
      841 gggacgccgc cctctacctt gcagaatggg ccatcgagaa cccggcagtc ttcactaaca
      901 ggactgtcct agagcttggc agtggtgctg gcctcacagg cctggccatc tgcaagatgt
      961 gccgcccc
//
