LOCUS       sequence-165897-        4493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW
  TITLE     cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis 
            elegans.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109.
  PUBMED    27992408
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109."
COMMENT     Sequence Label: pHW394 (15xUAS::GFP::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(868..896)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1644..1664
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2238..2342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2343..2411,2412..3203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2561..2580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3374..3962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3863..3882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4116..4133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4250..4271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4286..4291,4292..4309,4310..4316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4324..4340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4329..4351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4348..4364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4348..4364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      901 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      961 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
     1021 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
     1081 tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc
     1141 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1201 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     1261 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1321 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1381 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
     1441 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     1501 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1561 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1621 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1681 catggcatgg atgaactata caaatagcat tcgtagaatt cgctagccgg ccatacaagt
     1741 aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     1801 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     1861 attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc
     1921 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     1981 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     2041 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     2101 aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg
     2161 agaattgtcc tcgcttgggc ccaaaaggcc tttttttttt ggcgccgacg tcaggtggca
     2221 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2281 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2341 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2401 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2461 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2521 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2581 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2641 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2701 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2761 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2821 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2881 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2941 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3001 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3061 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3121 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3181 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3241 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3301 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3361 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3421 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3481 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3541 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3601 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3721 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4141 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4201 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4261 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4321 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     4381 tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa
     4441 aatggctgaa atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
