LOCUS       sequence-165898-        4819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct 
            for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding 
            domain (DBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW
  TITLE     cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis 
            elegans.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109.
  PUBMED    27992408
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109."
COMMENT     Sequence Label: pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'...
FEATURES             Location/Qualifiers
     source          1..4819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2026
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2564..2668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2669..2737,2738..3529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2887..2906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3700..4288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4189..4208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4442..4459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4576..4597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4612..4617,4618..4635,4636..4642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4650..4666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4655..4677
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4674..4690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4674..4690
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgatc ttcagatggg agcagtggac
       61 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
      121 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
      181 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
      241 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
      301 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
      361 aaaatctatt aaattatttc taaaatctct aaatatcgaa ttctctaaaa agcttcatca
      421 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
      481 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
      541 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
      601 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
      661 ggttgtgatg tatgcatcgg gcgtttctat ctctctccgt gagcaacgag ctagtcaacc
      721 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
      781 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
      841 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
      901 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
      961 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     1021 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     1081 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     1141 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     1201 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggcgcg cctctagagg
     1261 atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
     1321 aacattttca ggaggaccct tggctaggat gggccctaaa aagaagcgta aagctagcat
     1381 gaagctgttg tcttcaatgg aacaagcttg cgacatatgc cggttaaaaa aactcaggtg
     1441 ctcgaaggaa aagcccaagt gtgccaagtg tctgaagaac aactcggagt gccgttattc
     1501 tcccaaggct atgaggtccc cactgaccag ggcgcatctg acagaggtag aatcaaggtt
     1561 ggaaaaattg gaagatttgt ttctgctcat gtttcctcgg gaaaacctgg acagcatttt
     1621 gaacatggac tctttggacg aagtgaaagt aatgttgaaa caattatata tgcaggacta
     1681 tggtgacacg gatgctgcta tggatagctt gacccctatg gaaaatggca tgtctgaagg
     1741 gccgagaaaa cacatgaaga ctgcgacatc atcgccaaaa gtggatgaag atggaagcca
     1801 aagtcagttg agtgtatcgc cggaattccc gctgcagtct ggtggcggag gggctcctag
     1861 gggttccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
     1921 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
     1981 catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta actaaggtac
     2041 cgaattcgct agccggccat acaagtaatc cggatgatcg acgccaacgt cgttgaattt
     2101 tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt
     2161 tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatttt tttttctctt
     2221 tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat
     2281 cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa
     2341 aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg
     2401 gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa
     2461 taagcaaaga aattaggcga acgatgagaa ttgtcctcgc ttgggcccaa aaggcctttt
     2521 ttttttggcg ccgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2701 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2761 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2821 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2881 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2941 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3001 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3061 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3121 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3181 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3241 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3301 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3361 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3421 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3481 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3841 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4081 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4321 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4381 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4441 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4501 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     4561 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     4621 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     4681 cagctatgac catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat
     4741 tctcatttaa attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct
     4801 aacaacttgg aaatgaaat
//
