LOCUS       sequence-16590-e        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD50_14
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             549..569
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(659..681)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngcctgcgg tcgactctag aggatcaaga gcatttgaat cagaatatgg agaacggagc
       61 atgagcattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
      121 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
      181 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
      241 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
      301 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
      361 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaactata atcatctcac
      421 tggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
      481 tagaacattt tcaggaggac ccttggctag aggatccccg ggtaccgagc tcagaaaaaa
      541 tgactgctcc aaagaagaag cgtaaggtac cggtgggtga agaccagaaa cagcacctcg
      601 aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc gatcccgtcg
      661 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      721 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      781 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
      841 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
      901 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
      961 atccgccgtt tgttcccacg gagaatccga cgggttgnta ctcgctcnca tttaatgttg
     1021 atgaaagctg gctacaggaa ggccagacnc gaattatttt tgatggcgtt aactcggcgt
     1081 ttcntctgng gngcacgggc gctgggtcgn tacggcnaga nagtcnttgg ncgtctgaat
     1141 tgacngnagc gcattttncc ccccggaaaa acccccnccc ggggangggg ctncctgaat
     1201 gaccccnttn tcnggaaaan cagnattggg ggggagangg ggnttttccn ggacctcctt
     1261 tgggnaaaac nannacaaaa
//
