LOCUS       sequence-16591-e        1352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD50_21
FEATURES             Location/Qualifiers
     source          1..1352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             560..580
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(670..692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aatgngcctg cggtcgactc tagaggatca ccaaaaacgg aacgttgagc tggacggaaa
       61 tagtggtaaa gtgacatgat tatagtttga agatctctaa tttcacaatt agagcaaatg
      121 ttgttcggta tttattttca acggtattta tactattttc cacctttttc tagaacattc
      181 gagctgcttg ttgcaaaagg agggcgactc acattcggta catggaaaag tagtgtacac
      241 aataaagaga cccagataca ttttccgtct gcgtctcttt gcacccaccg ggagtatttt
      301 caaacgaatg catctaggac cttctagaac attctgtaag gctgcagaat gcgggtatat
      361 aaggaaagcg ggctcagagg aagccaacac gctttgttct agtgcatcta aaaaacttcg
      421 aaaatcctca tcggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
      481 ctaacataac atagaacatt ttcaggagga cccttggcta gaggatcccc gggtaccgag
      541 ctcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtgggtg aagaccagaa
      601 acagcacctc gaactgagcc gcgatattgc ccagcgtttc aacgcgctgt atggcgagat
      661 cgatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      721 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      781 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
      841 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
      901 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgactatccc
      961 attacggtca atccgccgtt ggtnccncgg agaatccgac gggttgttac tcnctcncat
     1021 ttatggtgat gaagctggct acaggaagnc nnacncaatt attttgangg cgtnactcgg
     1081 cgtttctctg ngngcacggg gctggtcggt acgncngnaa gtcnttgcng ctgaatngac
     1141 tgacgcattt taccnccgaa aaaccccncc ggggnggggc cccnggagnn cgcatttttg
     1201 gaaacagatg ggggagaggg ntttcggact cntgtgnnaa cnnacaaaaa gatncgngcc
     1261 ccctnagaat cccccntcgg gnnaataagg ggnggngnnn ccggaaattt tnggggaacc
     1321 nccccccccc nccggaaaaa angggntccc cc
//
