LOCUS       sequence-165923-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of redox sensitive GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pEGFP-N1-ro2GFP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..106,107..820)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(146..167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(407..426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     754..775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      181 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      241 catctccacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      301 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      361 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      421 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      481 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      541 ctgccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      601 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      661 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cctgctccgc
      721 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      781 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
      841 ataatcagcc ataccacatt tgtagaggtt
//
