LOCUS       sequence-165925-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as 
            a signal sequence that targets proteins for cytosolic translocation 
            via the co-administered protective antigen (PA) channel protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, 
            Kazmierczak BI, Vance RE
  TITLE     NAIP proteins are required for cytosolic detection of specific 
            bacterial ligands in vivo.
  JOURNAL   J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub
            2016 Apr 4.
  PUBMED    27045008
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20151809"; journalName: "J Exp Med"; date: "2016-05-2- 
            2"; volume: "213"; issue: "5"; pages: "657-65"
COMMENT     Sequence Label: pFastBacDual with LFn + Needle
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..156
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatcc
       61 cggtccgaag cgcgcggaat tcaaaggcct acgtcgacaa tgggcagcag ccatcatcat
      121 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggcgggcgg tcatggtgat
      181 gtaggtatgc acgtaaaaga gaaagagaaa aataaagatg agaataagag aaaagatgaa
      241 gaacgaaata aaacacagga agagcattta aaggaaatca tgaaacacat tgtaaaaata
      301 gaagtaaaag gggaggaagc tgttaaaaaa gaggcagcag aaaagctact tgagaaagta
      361 ccatctgatg ttttagagat gtataaagca attggaggaa agatatatat tgtggatggt
      421 gatattacaa aacatatatc tttagaagca ttatctgaag ataagaaaaa aataaaagac
      481 atttatggga aagatgcttt attacatgaa cattatgtat atgcaaaaga aggatatgaa
      541 cccgtacttg taatccaatc ttcggaagat tatgtagaaa atactgaaaa ggcactgaac
      601 gtttattatg aaataggtaa gatattatca agggatattt taagtaaaat taatcaacca
      661 tatcagaaat ttttagatgt attaaatacc attaaaaatg catctgattc agatggacaa
      721 gatcttttat ttactaatca gcttaaggaa catcccacag acttttctgt agaattcttg
      781 gaacaaaata gcaatgaggt acaagaagta tttgcgaaag cttttgcata ttatatcgag
      841 ccacagcatc gtgatgtttt acagctttat gcaccggaag cttt
//
