LOCUS       sequence-165933-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of LFn-Fla 3A fusion. This is a negative 
            control for LFn FlaA in which the 3 C-terminal leucines have been 
            replaced by alanine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, 
            Kazmierczak BI, Vance RE
  TITLE     NAIP proteins are required for cytosolic detection of specific 
            bacterial ligands in vivo.
  JOURNAL   J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub
            2016 Apr 4.
  PUBMED    27045008
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20151809"; journalName: "J Exp Med"; date: "2016-05-2- 
            2"; volume: "213"; issue: "5"; pages: "657-65"
COMMENT     Sequence Label: pET15b LFn-Fla 3A
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcgggc ggtcatggtg atgtaggtat gcacgtaaaa
      121 gagaaagaga aaaataaaga tgagaataag agaaaagatg aagaacgaaa taaaacacag
      181 gaagagcatt taaaggaaat catgaaacac attgtaaaaa tagaagtaaa aggggaggaa
      241 gctgttaaaa aagaggcagc agaaaagcta cttgagaaag taccatctga tgttttagag
      301 atgtataaag caattggagg aaagatatat attgtggatg gtgatattac aaaacatata
      361 tctttagaag cattatctga agataagaaa aaaataaaag acatttatgg gaaagatgct
      421 ttattacatg aacattatgt atatgcaaaa gaaggatatg aacccgtact tgtaatccaa
      481 tcttcggaag attatgtaga aaatactgaa aaggcactga acgtttatta tgaaataggt
      541 aagatattat caagggatat tttaagtaaa attaatcaac catatcagaa atttttagat
      601 gtattaaata ccattaaaaa tgcatctgat tcagatggac aagatctttt atttactaat
      661 cagcttaagg aacatcccac agacttttct gtagaattct tggaacaaaa tagcaatgag
      721 gtacaagaag tatttgcgaa agcttttgca tattatatcg agccacagca tcgtgatgtt
      781 ttacagcttt atgcaccgga agcttttaat tacatggata aatttaacga acaagaa
//
