LOCUS       sequence-165942-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pRosa26-EF1a-hCas9IRESneo
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          601..666
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctgggt acgccgcatc
       61 tagctagagt cgatcgacca gcttctgatg gaattagaac ttggcaaaac aatactgaga
      121 atgaagtgta tgtggaacag aggctgctga tctcgttctt caggctatga aactgacaca
      181 tttggaaacc acagtactta gaaccacaaa gtgggaatca agagaaaaac aatgatccca
      241 cgagagatct atagatctat agatcatgag tgggaggaat gagctggccc ttaatttggt
      301 tttgcttgtt taaattatga tatccaacta tgaaacatta tcataaagca atagtaaaga
      361 gccttcagta aagagcaggc atttatctaa tcccacccca cccccacccc cgtagctcca
      421 atccttccat tcaaaatgta ggtactctgt tctcaccctt cttaacaaag tatgacagga
      481 aaaacttcca ttttagtgga catctttatt gtttaataga tcatcaattt ctgcatccct
      541 cgactctagt ggatctgcat tccaccactg ctcccattca tcagttccat aggttggaat
      601 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
      661 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
      721 gtaaggttcc ttcacaaaga gatcgcctga cacgatttcc tgcacaggct tgagccatat
      781 actcatacat cgcatcttgg ccacgttttc cacgggtttc aaaattaatc tca
//
