LOCUS       sequence-16595-e        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD79_44
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..742
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(59..87)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     682..702
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(752..768)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(752..768)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(800..819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(801..819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(829..846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(829..845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggncaaagc tgggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt
       61 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      121 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      181 aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt
      241 ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
      301 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
      361 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      421 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
      481 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
      541 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      601 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
      661 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
      721 tggcatggat gaactataca aatgcccggg ggatccacta gttctagagc ggccgccacc
      781 gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      841 tttacaacgt cgtgactggg aaaacccctg gcgttaccca acttaatcgc cttgcagcac
      901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      961 agttgcgcag cctgaatggc gaatgggacn cgccctgtag cggcncatta ngcgcggcgg
     1021 ggggggtggt tacccgcagc gtgaccgctt accntggcng gngccnancn gcccgcccct
     1081 tnccttttct tcccttcctt tcncncccgn tcnccgggnt tncccggcag gcnctaaatc
     1141 gggggctcct ttagggnttc cnattagggc ttnccngccc nccaccccaa aacctgatag
     1201 gggngggtcc ntanggnccn ccccccgaaa acngntttcc ccccttgagg ggnaccccnt
     1261 tnttaanggg gacctntcca agggaaaccc cccccttccg ntttttttnt ttaaagnatt
     1321 gcntnccccc ttggaaaagg ggtttanaan tccgattnaa aantcccctt tggggcctnc
     1381 gggaggcccc cttt
//
