LOCUS       sequence-165967-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti virus delivery of LbCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY109 (lenti-LbCpf1)
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..43
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggtccggt gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
       61 agcagccagc aagctggaga agtttacaaa ctgctactcc ctgtctaaga ccctgaggtt
      121 caaggccatc cctgtgggca agacccagga gaacatcgac aataagcggc tgctggtgga
      181 ggacgagaag agagccgagg attataaggg cgtgaagaag ctgctggatc gctactatct
      241 gtcttttatc aacgacgtgc tgcacagcat caagctgaag aatctgaaca attacatcag
      301 cctgttccgg aagaaaacca gaaccgagaa ggagaataag gagctggaga acctggagat
      361 caatctgcgg aaggagatcg ccaaggcctt caagggcaac gagggctaca agtccctgtt
      421 taagaaggat atcatcgaga caatcctgcc agagttcctg gacgataagg acgagatcgc
      481 cctggtgaac agcttcaatg gctttaccac agccttcacc ggcttctttg ataacagaga
      541 gaatatgttt tccgaggagg ccaagagcac atccatcgcc ttcaggtgta tcaacgagaa
      601 tctgacccgc tacatctcta atatggacat cttcgagaag gtggacgcca tctttgataa
      661 gcacgaggtg caggagatca aggagaagat cctgaacagc gactatgatg tggaggattt
      721 ctttgagggc gagttcttta actttgtgct gacacaggag ggcatcgacg tgtataacgc
      781 catcatcggc ggcttcgtga ccgagagcgg cgagaagatc aagggcctga acgagtacat
      841 caacctgtat aatcagaaaa ccaagcagaa gctgcctaag tttaagccac tgtataagca
      901 ggtgctgagc gatcgggagt ctctgagctt ctacggcgag ggctatacat ccgatgagga
      961 ggtgctggag gtgtttagaa acaccctgaa caagaacagc gagatcttca gctccatcaa
     1021 gaagctg
//
