LOCUS       sequence-16597-e        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD81_34
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(245..267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngcctgcgg tcgactagag gatccccggg attggccaaa ggacccaaag gtatgtttcg
       61 aatgatacta acataacata gaacattttc aggaggaccc ttggagggta ccgagctcag
      121 aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac cagaaacagc
      181 acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc gagatcgatc
      241 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      301 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      361 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
      421 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
      481 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
      541 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
      601 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
      661 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
      721 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
      781 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
      841 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
      901 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
      961 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     1021 ccagcggcac ngcgcctttc ggnggngaan tatcgatgan cgnggnggtt atgcngatcg
     1081 cgtcccacta cgtctgaagg tcgaaaaccn naantgggga gngccnaaat cccnanttct
     1141 ttttnggggg ggtnaantgc ccccccccca ngncncctta tnaaacaaaa cctgnnannt
     1201 cgtttcccng agggngaatn aaantgnctg ntttgttnaa ggnaaccntt tttatnnagn
     1261 ttaccntcna aaaantcctt ggtggncggt tggnnaaaaa aangggnaga ttcttgaaaa
     1321 aaaaantnag cggggntttn tnanaccnct ggaacntgnc cccccggtng ggnaaaataa
     1381 nccnggggna attctca
//
