LOCUS       sequence-165970-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PGa
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     540..556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     540..556
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     561..580
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        561..579
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    598..829
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggcatgga tgaactatac aaatagcatt cgtagaattc cagctgagcg atcgcgcgcc
       61 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggggggat
      121 cgatccccca gatccgtacg atcctctagt aaaaaaaaaa caaaatttta gtgtgtataa
      181 ttaggctaaa caactggagg attcattgct ccctttgagt ttatactttt tgtaacaaga
      241 acaggcgcaa cactcgggag aattttgcat ttcatttgtg aataaacgaa tacaataaat
      301 acaaatacac gaaaagaagt cagctgtcga gtttttgttg gttttttttt ccctttttag
      361 cttcgaatct gcaaatgtat ttgcatttaa gtaaaccggc ttttgttggg tgtcgttaac
      421 tttcaagggg tcttatgtac ttattctgtt ttccttgtcc ttgaattgga cttgtggaaa
      481 attaaatggt cagccaattg cttaaccttt gtcgacctgc ggccgcggtg gccggccgcc
      541 aggaaacagc tatgaccatg taatacgact cactataggg gatatcagct ggatggcaaa
      601 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
      661 ttcttataat gccaactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata
      721 tcaatatatt aaattagact ttgcataaaa aacagactac ataatactgt aaaacacaac
      781 atatccagtc actatgaatc aactacttag atggtattag tgacctgtag tcgactaagt
      841 tggcagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg
      901 cagaataaat aaatcctggt gtcc
//
